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2 edition of Proteins involved in chromosome segregation in Kluyveromyces lactis found in the catalog.

Proteins involved in chromosome segregation in Kluyveromyces lactis

Aaron Adriaan Winkler

Proteins involved in chromosome segregation in Kluyveromyces lactis

by Aaron Adriaan Winkler

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Published by University of Leiden] in [Leiden .
Written in English


Edition Notes

StatementAaron Adriaan Winkler.
Classifications
LC ClassificationsMLCM 2002/02458
The Physical Object
Pagination105 p. :
Number of Pages105
ID Numbers
Open LibraryOL477073M
LC Control Number98202713

The similarity of the dosage compensation complex to condensin and the participation of MIX-1 in both complexes suggest a common mechanism for repressing X-chromosome gene expression during dosage compensation and for establishing chromosome resolution and higher order chromosome structure during mitotic and meiotic chromosome segregation.   The general field of fundamental and applied biotechnology becomes increasingly important for the production of biologicals for human and veterinary use, by using prokaryotic and eukaryotic microorganisms. The papers in the present book are refereed articles compiled from oral and poster presentations from the EFB Meeting on Recombinant Protein Production with Prokaryotic and /5(2).

  The RAG4 gene encodes for the sole transmembrane glucose sensor of Kluyveromyces lactis. A rag4 mutation leads to a fermentation-deficient phenotype (Rag− phenotype) and to a severe defect in the expression of the major glucose transporter gene RAG1. A recessive extragenic suppressor of the rag4 mutation has been identified. It encodes a protein (KlRgt1) 31% identical to the Cited by: Required for chromosome stability and methionine prototrophy. It is involved in chromosomal segregation. Binds to a highly conserved DNA sequence (5'-RTCACRTG-3'), called CDEI, found in centromeres and in several promoters (By similarity).

ID Protein Name Function 1 Function 2 Species Name; 3: SMC-3 (Structural maintenance chromosome 3), Mus musculus: SMC3 interacts with SMC1 and other non-Smc subunits like Scc3 and Scc1 (also called Rad21) to form a cohesion complex, called "cohesin," that maintains proper sister chromatid cohesion throughout the cell cycle and during mitosis to ensure accurate chromosome segregation. Abstract. The whole-genome duplication (WGD) that occurred during yeast evolution changed the basal number of chromosomes from 8 to However, the number of chromosomes in post-WGD species now ranges between 10 and the number in non-WGD species (Zygosaccharomyces, Kluyveromyces, Lachancea, and Ashbya) ranges between 6 and study the mechanism by which chromosome Cited by:


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Proteins involved in chromosome segregation in Kluyveromyces lactis by Aaron Adriaan Winkler Download PDF EPUB FB2

The Kluyveromyces lactis GAL4 gene contains a single UAS G element within its promoter region (, ). This element, found also in the promoters of lactose-inducible genes, can bind GAL4 and is necessary for full induction both of GAL4 synthesis and GAL4-dependent gene expression.

The fifth group includes proteins related to “transcription”, such as RNA polymerases and transcription factors. In the “cell division” group, proteins related to cell division such as chromosome segregation, budding and sporulation, were included.

The proteins involved in two-component systems are in the “signaling” : Lílian Cardoso de Paiva, Raphael Hermano Santos Diniz, Pedro Marcus Pereira Vidigal, Tiago Antônio d.

Lloret, A., A. Saviñón-Tejeda, L. Ongay-Larios, E. Tenorio, and R. Coria. The KlFUS1 gene is required for proper haploid mating and its expression is enhanced by the active form of the Gα (Gpa1) subunit involved in the pheromone response pathway of the yeast Kluyveromyces lactis.

FEMS Microbiol. Lett. Cited by: Abstract. Genetic studies of Kluyveromyces lactis (Dombrowski) van der Walt began in the early romyces lactis was the name of the yeast at that time.

Since then, the genus Kluyveromyces has been the object of intensive taxonomical studies. The position of Kluyveromyces lactis with respect to other members of the genus has been discussed in detail by a number of.

Abstract. In the yeast Kluyveromyces lactis heterologous proteins are efficiently produced and secreted. The amount of product formation, which is dependent on gene dosage, can be increased by copy number amplification of expression : Michele M. Bianchi. the protein- Kluyveromyces lactis Zip1.

Our studies reveal that although K. lactis Zip1 is unable to elongate and form stable structures along the full length of paired chromosomes in siae cells as siae Zip1 does, it is able to promote some degree of meiotic : Yashna Thappeta.

The gene bank of K. lactis MD2/1 was prepared on the basis of plasmid pBoriAMY by cloning the partially filled chromosomal DNA BspI fragments into the partially filled SalI site of kb URA3 cassette with S.

cerevisiae URA3 gene flanked by direct repeats of Salmonella hisG DNA (Alani et al., ) was used for both S. cerevisiae and K. lactis gene Cited by: Also involved in chromosome segregation where it has a role in chromosome attachment to the mitotic spindle (By similarity).

Protein knowledgebase. UniParc. Sequence archive. Help. Help pages, FAQs, UniProtKB manual, documents, news archive and Biocuration projects.

Kluyveromyces lactis (strain ATCC / CBS / DSM / NBRC. Introduction. The milk yeast Kluyveromyces lactis is of industrial importance as a producer of β-galactosidase and has also been widely used as a host for heterologous protein production (van Ooyen, ).The completion of the genome sequence of the K.

lactis type strain CBS has further stimulated research in all areas of molecular genetics in this organism (Dujon, ).Cited by: 6 Kluyveromyces lactis: genetics, physiology, and application 21 in the LIPB gene, encoding a lipoyl-pr otein ligase involved i n the lipoylation of proteins (Chen ).

9 Centromere and Kinetochore: Essential Components for Chromosome Segregation contain a GC-rich satellite sequence along with retroelement cerebra, spanning up to Mb in length [85]. Abstract Two genes of Kluyveromyces lactis, KlPDI1 and KlMPD1, were studied.

They code for a protein disulphide isomerase and its structural and functional Cited by: Finally, consistent with this condensation-resolution scheme, bulk chromosome segregation is impaired when the proteins and processes that function in chromosome compaction are compromised.

Specifically, cells with defects in supercoiling or that lack the small nucleoid-associated proteins or components of the SMC complex have segregation defects characterized by the formation of Cited by: The translocations were verified by Southern blotting using probes against the right and left ends of both chromosomes involved in the translocation (n = 4 different probes).

A representative. Although the recently discovered cytoskeleton-like structures within bacterial cells (some of which are involved in plasmid segregation) raise the possibility that similar structures might be involved in chromosome segregation [18••••], the observation that DNA polymerase is a motor protein which resides in a stationary replisome, has led to the ‘extrusion-capture’ model for chromosome segregation Cited by: Sequence analysis of a DNA fragment containing the KlCOX18 gene originating from chromosome II of the yeast Kluyveromyces lactis revealed the presence of an adjacent open reading frame (ORF) for a.

Required for high-fidelity chromosome segregation during the later part of each cell cycle. Acts in opposition to the phosphatase PP1. Has a role in attaching the kinetochores to the microtubules and ensuring that sister kinetochores connect to opposite poles.

The promotion of bi-orientation is achieved by selectively detaching kinetochore-microtubule attachments that are not under tension. Component of the kinetochore, a multiprotein complex that assembles on centromeric DNA and attaches chromosomes to spindle microtubules, mediating chromosome segregation and sister chromatid segregation during meiosis and mitosis (By similarity).

Component of the inner kinetochore COMA subcomplex, which connects centromere-associated proteins and the outer kinetochore. Synthetic biology and molecular genetics in non-conventional yeasts: Current tools and future advances Kluyveromyces lactis, Pichia pastoris, and Yarrowia lipolytica.

These yeasts already have roles in the manufacture of vaccines, therapeutic proteins, food additives, and biorenewable chemicals, but recent synthetic biology advances have Cited by: K. lactis HMLalpha contained three genes, the alpha1 and alpha2 genes, which were similar to their Saccharomyces counterparts, and a novel third gene, alpha3.

A dam-methylase assay showed Sir-dependent, but transcription-independent changes of the chromatin structure of the HMLalpha by:. A Protein Homologous to Csm1 (Pcs1) Is Located at Centromeres and Nucleoli in S.

pombe and Is Involved in Chromosome Segregation We identified in genome databases Csm1-like proteins from S. pombe, Neurospora crassa, and Candida albicans (Figures Cited by: in chromosome segregation and transcription activation we decided to study this protein in Kluyveromyces lactis.

This yeast is related to S. cerevisiae and generally shows a high conservation of.Introduction. The segregation of homologous chromosomes at meiosis I is essential for the formation of haploid reproductive cells.

Accurate segregation is dependent on the establishment of one or more associations between homologous chromosomes [1,2].For most organisms, crossover recombination events in conjunction with sister chromatid cohesion provide the temporary associations needed Cited by: